Uptake of Vitamin B_6 by Ascites-Sarcoma Cells : (I) UPTAKE OF PYRIDOXINE

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  • TSUJI Minoru
    Department of Internal Medicine, Nagoya University School of Medicine
  • NISHIWAKI Jun
    Department of Internal Medicine, Nagoya University School of Medicine
  • YAMADA Kozo
    Department of Internal Medicine, Nagoya University School of Medicine

Bibliographic Information

Other Title
  • ビタミンB_6の腹水腫瘍細胞へのとりこみ : (I)ピリドキシンのとりこみ
  • ピリドキシンのとりこみ
  • ピリドキシン ノ トリコミ

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Description

Uptake of pyridoxine by ascites-sarcoma cells in vitro has been investigated. Pyridoxine was uptaken against concentration gradient at low initial concentrations of pyridoxine and accumulated in the cells as phosphorylated forms of B_6. The uptake of pyridoxine by ascites-cells was greatly enhanced in the presence of glucose but it was diminished by the addition of either 2,4-dinitrophenol or sodium fluoride with glucose. 4-Deoxypyridoxine also inhibited the uptake of pyridoxine in the cells. Incubation of ascites-cells with glucose and labelled pyridoxine resulted in accumulation of labelled pyridoxine in the cells. Similarly, the accumulation was decreased by the addition of each 2,4-dinitrophenol or sodium fluoride. Furthermore, in the presence of vitamin B_6 phosphates and 4-deoxypyridoxine, labelled pyridoxine uptake was inhibited. These results support the conclusion that pyridoxine was uptaken in ascites-sarcoma cells by an energy-associated process.

Journal

  • VITAMINS

    VITAMINS 42 (4), 222-228, 1970

    THE VITAMIN SOCIETY OF JAPAN

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