Measurement of 6-Aminonicotinamide, an Antagonist of Niacin, by High-performance Liquid Chromatography

  • SHIBATA Katsumi
    Department of Human Health Science, Faculty of Human Sciences, Osaka International University for Women

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Other Title
  • ニコチンアミドの拮抗体,6-アミノニコチンアミドの高速液体クロマトグラフを利用する定量方法
  • ニコチンアミド ノ キッコウタイ 6 アミノニコチンアミド ノ コウソク エキ

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Abstract

The author has developed the HPLC measurement of 6-aminonicotinamide, which is the most powerful antagonist of niacin. The analytical method for the measurement of 6-aminonicotinamide used a 7-ODS-L column (250 mm × 4.6 mm I. D. , particle size 7 μm) eluted with 10 mM potassium dihydrogenphosphate-acetonitrile (96 : 4, v/v, pH was adjusted to 3.0 by addition of concentrated phosphoric acid) at a flow-rate of 1.0 ml/min. The UV detector was set at 266 nm. The column temperature was maintained at 25℃. Under the conditions, 6-aminonicotinamide was eluted at about 4 min and the simultaneous measurements of nicotinamide, N^<-1>-methyl-2-pyridone-5-carboxamide and N^<-1>-methyl-4-pyridone-3-carboxamide were possible. The detection limit was 4 pmol at a signal-to-noise ratio of 5 : 1. The total time required for one analysis was around 15 min. The 6-aminonicotinamide in the liver and urine of the rats fed with a diet being added 6-aminonicotinamide was measured as test food samples containing 6-aminonicotinamide.

Journal

  • VITAMINS

    VITAMINS 67 (9), 493-497, 1993

    THE VITAMIN SOCIETY OF JAPAN

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