Studies on Mammalian Lipoxygenases : II. Development of Selective Inhibitor and Enzyme Immunoassay

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  • YAMAMOTO Shozo
    Department of Biochemistry, Tokushima University, School of Medicine
  • YOSHIMOTO Tanihiro
    Department of Biochemistry, Tokushima University, School of Medicine
  • UEDA Natsuo
    Department of Biochemistry, Tokushima University, School of Medicine
  • EHARA Hiroaki
    Department of Biochemistry, Tokushima University, School of Medicine

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Other Title
  • 動物組織のリポキシゲナーゼの研究 : II.特異的阻害剤と酵素免疫測定法の開発
  • 動物組織のリポキシゲナーゼの研究-2-特異的阻害剤と酵素免疫測定法の開発
  • ドウブツ ソシキ ノ リポキシゲナーゼ ノ ケンキュウ 2 トクイテキ ソガイ

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Abstract

In view of pathological roles of leukotrienes a potent selective inhibitor of arachidonate 5-lipoxygenase was developed on the basis of an earlier finding that flavonoids suppressed anaphylactic reactions. 5-Hexyloxy-3',4'-dihydroxy-6,7-dimethoxyflavone 4'-disodium phosphate as a 5-lipoxygenase inhibitor inhibited in vivo production of peptidoleukotrienes in the gerbil brain subjected to ischemia and reperfusion. The monoclonal antibodies raised against 12- and 5-lipoxygenases and cyclooxygenase were utilized for development of peroxidase-linked immunoassays of these enzymes, which allowed a quantitative determination of the enzyme amount rather than the enzyme activity. The method was applied to lipoxygenase screening in various animal tissues. A clinical case of cyclooxygenase abnormality was analyzed by the enzyme immunoassay.

Journal

  • VITAMINS

    VITAMINS 62 (2), 61-67, 1988

    THE VITAMIN SOCIETY OF JAPAN

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