Flavobacterium sp.によるビタミンB_6の生合成

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  • 谷 吉樹
    京都大学農学部生物細胞生産制御実験センター

書誌事項

タイトル別名
  • Biosynthesis of Vitamin B_6 by Flavobacterium sp.
  • Flavobacterium sp.ニ ヨル ビタミン B6 ノ セイゴウセイ

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説明

Flavobacterium sp. 238-7 excreted a considerably high amount of vitamin B_6 i.e. 20 μg/ml, in the form of pyridoxine into the culture medium. The cellular productivity was higher than that of other microorganisms. A resting cell system of vitamin B_6 biosynthesis was developed. In screening of compounds effective for improvement of the vitamin production, some nucleosides and nucleotides were found to have a strong stimulating effect on the biosynthesis. Some amino acids were also effective. Though the addition of glycolaldehyde did not increase the amount of vitamin B_6 in the system, a significant enzyme activity for reduction of glycolate to glycolaldehyde was found in a membrane fraction of the cell free extract. The enzyme catalyzed the reduction but not the oxidation, namely the reverse reaction, and was therefore designated as glycolate reductase. A positive correlation was observed between the enzyme activity and the biosynthesis of vitamin B_6 during the cultivation. The high vitamin productivity of the bacterium could be explained by lack of regulation of vitamin B_6 biosynthesis in the growing and resting cell systems and by that of glycolate reductase.

収録刊行物

  • ビタミン

    ビタミン 58 (9-10), 409-419, 1984

    公益社団法人 日本ビタミン学会

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