Sex discrimination in the sika deer (Cervus nippon) using a hair root by the LAMP method

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  • Yamada Shinya
    Forestry and Forest Products Research Center, Shizuoka Prefectural Research Institute of Agriculture and Forestry
  • Yamaguchi Akira
    Forestry and Forest Products Research Center, Shizuoka Prefectural Research Institute of Agriculture and Forestry
  • Ishikawa Keisuke
    Forestry and Forest Products Research Center, Shizuoka Prefectural Research Institute of Agriculture and Forestry
  • Maki Fuminori
    NIPPON GENE CO., LTD.
  • Ohtake Masayoshi
    Forestry and Forest Products Research Center, Shizuoka Prefectural Research Institute of Agriculture and Forestry
  • Ohashi Masataka
    Forestry and Forest Products Research Center, Shizuoka Prefectural Research Institute of Agriculture and Forestry
  • Katai Yusuke
    Forestry and Forest Products Research Center, Shizuoka Prefectural Research Institute of Agriculture and Forestry
  • Ohba Takahiro
    Forestry and Forest Products Research Center, Shizuoka Prefectural Research Institute of Agriculture and Forestry

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Other Title
  • LAMP法を用いた毛根からのニホンジカ雌雄判別法
  • LAMPホウ オ モチイタ モウコン カラ ノ ニホンジカ シユウ ハンベツホウ

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Abstract

We developed a technique to determine the sex of the sika deer (Cervus nippon) using its hair root by the LAMP method. The SRY gene on the Y chromosome and ZFX gene on the X chromosome were used for sex discrimination. If these two genes were amplified and colored, the deer was a male, and if only the ZFX gene was amplified and colored, it was a female. In order to efficiently amplify the target gene from the hair root of the sika deer, we investigated the reaction conditions. The LAMP method was performed at 64℃ for 40 min. We showed that the LAMP method could be used to accurately identify the sex of this Japanese deer, regardless of strain differences.

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