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- KIMURA Takao
- Waseda University Tokyo Women Medical University
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- SEKINE Hidekazu
- Tokyo Women Medical University
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- SANO Kazunori
- Tokyo Women Medical University Tokaihit Co, Ltd.
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- SHIMIZU Tatsuya
- Tokyo Women Medical University
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- UMEZU Shinjiro
- Waseda University
Bibliographic Information
- Other Title
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- 心筋収縮力測定器の開発
Abstract
<p>Thick three-dimensional tissue engineered structures have a great future potential in various areas of regenerative medicine. However, the diffusion limit of nutrients, oxygen and metabolites represents a major challenge. The constructs consist of stacked human cardiac cell sheets which are vascularized through tissue engineered vascular beds. Conventionally, stacking cell sheets are limited due to cell necrosis caused by lack of oxygen or nutrition. Therefore, in order to create thick tissues, it is necessary to create vascular networks in the stacked cell sheets and perfuse them with cell culture media. The purpose of this study is to create thick myocardial implantable and functional tissues with pre-existing vascular networks and make mechanical evaluation of the cardiac tissue. We created vascular beds from porcine small intestinal tissue by trimming it into a flat surface, removing mucosa and finally sterilizing it with peracetic acid. Triple-layers of human cardiac cell sheets were then stacked using the stamp method. We performed mechanical analyses using a newly developed, 3D-printed device capable of real time measurement of contraction force during cardiac tissue culture. Cell sheet morphology was assessed using optical coherence tomography. Real time contractile force could successfully be recorded using the newly developed device.</p>
Journal
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- The Proceedings of Mechanical Engineering Congress, Japan
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The Proceedings of Mechanical Engineering Congress, Japan 2017 (0), J1630101-, 2017
The Japan Society of Mechanical Engineers
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Details 詳細情報について
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- CRID
- 1390282680820525952
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- NII Article ID
- 130006550421
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- ISSN
- 24242667
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- Text Lang
- ja
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed