In-Situ Observation of Diffusion, Adsorption and Desorption Processes of Individual Protein Molecules on Protein Crystal Surfaces(<Special Issue>The Forefront of Protein Crystal Growth)

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  • Sazaki Gen
    Institute for Materials Research, Tohoku University:(Present office)Institute of Low Temperature Science, Hokkaido University
  • Okada Masashi
    Institute for Materials Research, Tohoku University:(Present office)Konica Minolta Business Technologies, Inc.
  • Dai Guoliang
    Institute of Mechanics, Chinese Academy of Science
  • Matsui Takuro
    Institute for Materials Research, Tohoku University
  • Tsukamoto Katsuo
    Graduate School of Science, Tohoku University
  • Nakajima Kazuo
    Institute for Materials Research, Tohoku University:(Present office)Graduate School of Energy Science, Kyoto University

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  • タンパク質結晶表面上でのタンパク質分子の拡散・吸着・脱離挙動の光学その場観察(<特集>タンパク質結晶成長の最前線)
  • タンパク質結晶表面上でのタンパク質分子の拡散・吸着・脱離挙動の光学その場観察
  • タンパクシツ ケッショウ ヒョウメン ジョウ デ ノ タンパクシツ ブンシ ノ カクサン ・ キュウチャク ・ ダツリ キョドウ ノ コウガク ソノ バ カンサツ

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We observed in-situ individual fluorescence-labeled protein molecules, to reveal the dynamic behavior of protein molecules during diffusion, adsorption and desorption at an interface between a protein solution and a protein crystal. We found that protein molecules diffused along a crystal surface 4-5 orders of magnitude slower than in a bulk solution, indicating that the molecules strongly interacted with molecules that were aligned at the crystal surface. This result denotes that slow two-dimensional diffusion inside a range of interactions from the crystal surface is a general picture of surface diffusion at an interface between an aqueous solution and a hydrophilic crystal surface. We also found the existence of an induction period (〜70 min) after which the number density of protein molecules adsorbed on steps increased linearly with the adsorption time. We show direct evidences that the residence time of molecules on a crystal surface gradually increases during the transition process from a solute species to the crystal after successive multistep processes.

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