Safety Assessment of Refuse-derived Fuel by Combination of Meta-genome Analysis with Cultivation Method

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  • メタゲノム解析と培養方法を組み合わせたごみ固形燃料の安全性評価
  • メタゲノム カイセキ ト バイヨウ ホウホウ オ クミアワセタ ゴミ コケイ ネンリョウ ノ アンゼンセイ ヒョウカ

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This research analyzes Refuse-derived-fuel (RDF) produced in different plants from five areas of Mie Prefecture, and focuses on the fermentation of organic substances inducing the production of flammable gases, and considered to be the major factor of Mie RDF fires. In this analysis, cultivation experiments and meta-genome analysis were used. In RDF manufactured by the RMJ system, spore-forming bacteria, such as Bacillus sp. and Clostridium sp., and heat-resistant bacilli, such as B.thermoamylovorans, survived under 230 - 280 ℃ hot wind, and were proliferated by added moisture. Among Aerobe and Anaerobe facultative, which can be multiplied with oxygen existence after cultivation starts, the species that survived were as follows; B.circulans which can be cultivated under high pH environment, as well as Sphingomonas sp., B.coagulans, and B.subtilis which can be cultivated under middle-low pH environment. The multiplication of bacteria and the decline of oxygen concentration are affected by early stage pH levels of RDF. In regards to Hydrogen producing bacillus that exist under low Oxygen, the following were found: B.coagulans, B.licheniformis, and C.tertium in Kuwana; C.acetobutylicum, C. beijerinckii, C. intestinale, C. paraputrificum, C. perfringens, and C.roseum in Sakura. It is considered that most bacilli that proliferated were those adaptable to various pH levels. It is considered that the decline of hydrogen concentration at Hamajima was caused by non Hydrogen producing Lysinibacillus sphaericus.

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