書誌事項
- タイトル別名
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- Viability of Densely Cultured Artificial Tissue in Collagen Matrix after Cryopreservation
抄録
The effect of cell density on the post-thaw viability of cells in cryopreserved artificial tissue was studied. Human fibroblasts were three-dimensionally cultured for 2 days in collagen sponge (Φ20×1mm) as an extracellular matrix to imitate biological tissue (artificial tissue). Different cell densities for the artificial tissue were used, from (10)^4 to (10)^7 cells/(cm)^3. Four artificial tissues were first stacked in a test chamber, then frozen at a slow or fast cooling rate (either 1 or 50℃/min) in a solution of Dulbecco's Modified Eagle Medium, 20% Fetal Bovine Serum, and 10% dimethylsulfoxide, then kept frozen at -196℃ for 2 hours, and finally thawed. Collagen matrix of artificial tissue was resolved by using collagenase. Post-thaw viability of fibroblasts was evaluated by using a trypan blue exclusion assay. The experiments were prepeated, and then the latent heat of artificial tissue (3×3×1mm) during the freeze-thaw process was measured by using a differential scanning calorimeter. Results show that with increasing cell density, the post-thaw viability decreased, whereas the latent heat was constant. With increasing cell density at the slow cooling rate, temperature of the peak latent heat decreased and and the accumulated fraction of latent heat significantly increased, possibly leading to intracellular freezing. Moreover, when the cell density was high, dehydration was obstructed, and thus the number of fibroblasts exhibiting intracellular freezing increased, which in turn led to an increase in latent heat. Therefore, the post-thaw viability decreased as the number of cells exhibiting intracellular freezing increased.
収録刊行物
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- 年次大会講演論文集
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年次大会講演論文集 2000.2 (0), 271-272, 2000
一般社団法人 日本機械学会
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詳細情報 詳細情報について
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- CRID
- 1390282681035889536
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- NII論文ID
- 110002522735
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- ISSN
- 24331325
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
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- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可