12 Enantio-Differential Approach to the Receptor Protein of glucosylated Jasmonate-type leaf-closing factor
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- Nakamura Yoko
- Department of Chemistry, Tohoku University
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- Miyatake Ryoji
- Department of Chemistry, Tohoku University
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- Inomata Sho
- Department of Chemistry, Tohoku University
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- Kiyota Hiromasa
- Department of Applied Bioorganic Chemistry, Tohoku University
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- Ueda Minoru
- Department of Chemistry, Tohoku University
Bibliographic Information
- Other Title
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- 12 エナンチオ・ディファレンシャル法によるジャスモン酸配糖体型就眠物質受容体タンパク質の生物有機化学(口頭発表の部)
Abstract
Albizzia plants close their leaves in the evening, as if to sleep, and open them in the morning according to the circadian rhythum. Potassium β-D-glucopyranosyl-12-hydroxyjasmonate was isolated as leaf-closing factor (LCF) of Albizzia saman. We developed molecular probes consisting of modified LCF in order to identify its mode of action. However, it is often discussed whether molecular probes containing a larger and less polar functional unit such as fluorescence dye can bind to genuine binding protein of a natural product in living organisms. So, we need appropriate negative control. And an enantiomer of a biologically active natural product can be used as an ideal control because all physical propaties except for optical rotation and affinity to binding proteins are identical between a pair of enantiomers. Thus, enantio-differential approach that compares the results using a pair of probes that was prepared from a pair of enantiomers would be an ideal method in the bioorganic study using molecular probes. We synthesized probe 11 and biologically inactive enantiomer-type probe 12. And carried out fluorescence labeling study using 11 and 12. The results was clearly shown that the motor cells contain some specific binding protein that can differentiate the stereochemistry of the ligands. Similarly, we synthesized enantio pair-type photoaffinity-labeling probes 20 and 21, and used them for photoaffinity labeling of receptor for LCF. By using protoplast of motor cell, we found 35kDa membrane protein strictly recognizes the stereochemistry of LCF, and it is highly likely that the protein is the specific receptor for LCF.
Journal
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- Symposium on the Chemistry of Natural Products, symposium papers
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Symposium on the Chemistry of Natural Products, symposium papers 49 (0), 67-72, 2007
Symposium on the Chemistry of Natural Products Steering Committee
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Details 詳細情報について
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- CRID
- 1390282681056345856
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- NII Article ID
- 110006682742
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- ISSN
- 24331856
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- Text Lang
- ja
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed