甲殻類の染色体標本簡易作製法

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書誌事項

タイトル別名
  • SIMPLE AND RAPID TECHNIQUE FOR A CHROMOSOME STUDY OF CRUSTACEA
  • コウカクルイ ノ センショクタイ ヒョウホン カンイ サクセイホウ

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説明

A simple technique is described for obtaining well-spread metaphases from solid tissues of Crustacer. The procedure of chromosome preparation was as follows : 1) Specimens were injected intraperitoneally with colchicine solution at the dosage of I pg per 1μg body weight five hours before being dissected. 2) Remove antennal gland and testis, and cut them into 3mm pieces where necessary. 3) Place tissue pieces in the hypotonic solution (distilled water or 0.01M KCl) for 10 minutes. 4) Add small quantity of Carnoy's fluid (3:1 ethanol acetic acid). 5) Fix in at least two changes of Carnoy's fluid for at least 15 minutes each. 6) Pick tissue pieces up from Carnoy's fluid by a forceps, and tap to spread them on a slide glass for a proper density of cells. 7) Drop Carnoy's fluid on the slide from a fine pipet. 8) Dry quickly by flaming. 9) Stain with 2% Giemsa for 15 minutes. Large numbers of slides of chromosome can be made easily and rapidly using this procedure.

収録刊行物

  • 甲殻類の研究

    甲殻類の研究 11 (0), 110-112a, 1981

    一般社団法人 日本甲殻類学会

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