Chemical Profiles of Methylpyrazines Contained in Commercially Available Natto
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- KANUMA Midori
- <i>Department of Clinical Pharmacology, School of Pharmacy, Tokyo University of Pharmacy and Life Science</i>
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- YAMADA Chie
- <i>Department of Clinical Pharmacology, School of Pharmacy, Tokyo University of Pharmacy and Life Science</i>
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- OKA Kitaro
- <i>Department of Clinical Pharmacology, School of Pharmacy, Tokyo University of Pharmacy and Life Science</i>
Bibliographic Information
- Other Title
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- 納豆に含まれているメチルピラジン類のHPLCによる分析
- ナットウ ニ フクマレテ イル メチルピラジンルイ ノ HPLC ニ ヨル ブンセキ
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Description
Structures and amounts of methylpyrazines contained in commercial natto, a fermented soybean food in Japan, were determined using HPLC equipped with an acid-resistant reversed phase column, Capcell Pak C18 ACR (Shiseido). Mobile phase solvent mixtures consisted of acidic phosphate buffer solution (pH 2.0) containing 2% acetonitrile gave satisfactory results with baseline separation of the authentic specimens, such as naked pyrazine, monomethylpyrazine, 2,3-, 2,5-, and 2,6-dimethylpyrazine, trimethylpyrazine, and tetramethylpyrazine. We used the mobile-phase solvent with a flow rate of 1 ml/min at 15.0°C. Before HPLC, commercial natto samples were treated with water to prepare diluted suspensions of surface mucous materials. The suspensions were treated on Sep-Pac C18 Cartridges (Waters) with phosphate buffer solutions containing 2—7% acetonitrile. The extracts were then injected into the analytical column to obtain chromatograms that were used to determine the structures and amounts of methylpyrazines. The results showed that a commercialiy packed natto contains a considerable amount of 2,5-dimethylpyrazine instead of the tetrametyl- and trimethylpyrazines in the traditional products. This may be a result of recent efforts of natto makers whose interests have been focused on new methods for preparing odorless products.
Journal
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- YAKUGAKU ZASSHI
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YAKUGAKU ZASSHI 124 (1), 31-36, 2004-01-01
The Pharmaceutical Society of Japan
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Details 詳細情報について
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- CRID
- 1390282681103569664
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- NII Article ID
- 110003614934
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- NII Book ID
- AN00284903
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- ISSN
- 13475231
- 00316903
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- NDL BIB ID
- 6810531
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- PubMed
- 14768353
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed