速乾性擦式アルコール手指消毒剤の皮膚刺激性評価における動物実験代替法の開発

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  • 山本 宣之
    日油株式会社ライフサイエンス事業部筑波研究所第 2 研究室
  • 宮本 幸治
    日油株式会社ライフサイエンス事業部筑波研究所第 2 研究室
  • 加藤 雅一
    株式会社ジャパン・ティッシュ・エンジニアリング研究開発部

書誌事項

タイトル別名
  • Development of Alternative to Animal Experiment in Evaluation of Skin Irritation Caused by Alcohol-based Hand Rubs
  • ソッカンセイ サッシキ アルコール シュシ ショウドクザイ ノ ヒフ シゲキセイ ヒョウカ ニ オケル ドウブツ ジッケン ダイタイホウ ノ カイハツ

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抄録

  Alcohol-based hand rubs are widely used for infection control in clinical practice. However, it is known that frequent use of the alcohol-based hand rubs may cause skin irritation. To predict the skin irritation in human, animal experiments are quite useful. Especially, the Draize Test using rabbits is suitable for this purpose because their skin is highly sensitive. On the other hand, the development of alternative to animal experiments is important not only from the viewpoint of ethical aspects but also from the efficient research and development. Reconstructed human epidermis (RhE) was developed as a human skin equivalent model in vitro, and has been applied to the evaluation of skin irritation. But the RhE has not been utilized for the evaluation of alcohol-based hand rubs because of the high skin permeability and cytotoxicity of alcohols. The aim of this study was to develop a new method using the RhE in evaluation of skin irritation caused by alcohol-based hand rubs. The authors propose an experimental technique named “Skin model blowing method (SMBM)” consisting of the sequential procedure as follows; applying small amount of testing sample on RhE, blow-dry, post incubation, and cell viability measurement. According to the SMBM, the skin irritation caused by alcohol-based hand rubs could be evaluated under the similar condition of their actual use. It was found that a high correlation existed between the cell viability obtained from SMBM and the skin irritation index in rabbit which had been reported previously.<br>

収録刊行物

  • 薬学雑誌

    薬学雑誌 130 (8), 1069-1073, 2010-08-01

    公益社団法人 日本薬学会

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