Estrogenic Activity of Ultraviolet Absorbers and the Related Compounds

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  • MATSUMOTO Hisashi
    Department of Food and Drugs, Osaka Prefectural Institute of Public Health
  • ADACHI Shinichi
    Department of Environmental Health, Osaka Prefectural Institute of Public Health
  • SUZUKI Yasuhiko
    Department of Microbiology and Pathology, Faculty of Medicine, Tottori University

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Other Title
  • 紫外線吸収剤及びその関連化合物によるエストロゲン様作用
  • シガイセン キュウシュウザイ オヨビ ソノ カンレン カゴウブツ ニ ヨル エストロゲン ヨウ サヨウ

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  The estrogenic activities of ultraviolet absorbers and their related compounds were investigated using MCF-7 cell proliferation assay. Nine of 33 chemicals (benzophenone, 2,4-dihydroxybenzophenone, 2,2′,4,4′-tetrahydroxybenzophenone, 2-hydroxy-4-methoxybenzophenone, 2,2′-dihydroxy-4,4′-dimethoxybenzophenone, 4-hydroxybenzophenone, 3-(4-methylbenzylidene) camphor, ethyl 2-cyano-3,3-diphenylacrylate (etocrylene) and 2-ethylhexyl-2-cyano-3,3-diphenylacrylate (octocrylene)) were positive compared with the vehicle control. Benzhydrol, ethyl cinnamate and 2,2′-dihydroxy-4-methoxybenzophenone were weakly active. When each xenoestrogen was added to the cells along with ICI 182780, an estrogen receptor (ER) antagonist, the cell growth was reduced according to its doses. Therefore, the cell proliferation was suggested to generate through ER. Most of these chemicals were also positive using CHOOSER assay, a new method of testing estrogenic activity of xenoestrogen. Each xenoestrogen was also confirmed to bind to ERα and ERβ using a human ER competitive binding assay against 17β-estradiol. The concentration order of the strength of its inhibitory effect using both ERα and ERβ was similar to that of MCF-7 cell proliferation assay, except for benzyl 4-hydroxybenzoate (B4HB). B4HB showed a stronger activity on CHOOSER assay and the competitive binding assay using both ERα and ERβ, although there was no activity observed on MCF-7 cell proliferation assay. Our findings were to detect the estrogenic activity of etocrylene and octocrylene in vitro, in addition to confirming the activities of some ultraviolet absorbers as previously reported.<br>

Journal

  • YAKUGAKU ZASSHI

    YAKUGAKU ZASSHI 125 (8), 643-652, 2005-08-01

    The Pharmaceutical Society of Japan

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