後眼部指向型核酸封入リポソーム点眼剤の設計

DOI Web Site Web Site PubMed 被引用文献1件 参考文献11件

書誌事項

タイトル別名
  • Non-Invasive Ophthalmic Liposomes for Nucleic Acid Delivery to Posterior Segment of Eye
  • ゴガンブ シコウガタ カクサン フウニュウ リポソーム テンガンザイ ノ セッケイ

この論文をさがす

抄録

  Nucleic acids like siRNA and pDNA are remarkable for treatment of ophthalmic diseases in posterior segment of eye such as age-related macular degeneration (AMD). However, hydrophilic and high molecule compounds are restricted in intraocular distribution through anterior segment of the eye. In addition, the ocular tissue has a blood-retinal barrier which restricts drug delivery thorough systemic administration. Therefore the invasive intravitreal injection has been generally applied for treatment of retinal diseases. The objective in this study is to prepare nucleic acid-loaded liposomes for effective gene delivery to posterior segment of eye by non-invasive ophthalmic administration such as eye-drops. The pDNA/PEI-complex loaded liposomes were prepared using detergent removal method. The obtained liposomes were lyophilized with optimal amount of a cryoprotectant to avoid changes in physical properties and, followed by adjustment of an appropriate volume and osmotic pressure as ophthalmic solution. The liposomes show high pDNA encapsulation efficiency and good cellular uptake ability in human retinal pigment epithelial cells (ARPE-19 cells). We further demonstrate that the modification of ligand which binds to specific receptor on the RPE cells to the liposomes may improve gene delivery efficacy to the posterior segment of eye by non-invasive ocular instillation.<br>

収録刊行物

  • 薬学雑誌

    薬学雑誌 132 (12), 1365-1370, 2012-12-01

    公益社団法人 日本薬学会

被引用文献 (1)*注記

もっと見る

参考文献 (11)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ