ヒト乳がん由来 MCF-7 細胞を用いた食品添加物等の内分泌かく乱作用の検索と BHA, OPP を中心とした機構の解析

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タイトル別名
  • Studies on Estrogenic Activities of Food Additives with Human Breast Cancer MCF-7 Cells and Mechanism of Estrogenicity by BHA and OPP
  • ヒト ニュウガン ユライ MCF 7 サイボウ オ モチイタ ショクヒン テンカブツ トウ ノ ナイブンピカク ランサヨウ ノ ケンサク ト BHA OPP オ チュウシン ト シタ キコウ ノ カイセキ

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Estrogenic activities of more than 90 chemicals including food additives, foodstuffs of plant origin, and some chemicals, which could be orally ingested, were examined by assaying estrogen receptor (ER)-dependent proliferation of MCF-7 cells. Among 66 food additives, 17 compounds stimulated the proliferation, but their concentrations giving maximal cell yield were higher than that of 17β-estradiol and their estrogenic activities were weak. Flavonoids had relatively strong estrogenic activities. In the assay of ER competitive binding to human ERα and ERβ in vitro, the antioxidant t-butylhydroxyanisole (BHA) had the capacity to compete with 17β-estradiol, while the capacity of o-phenyl phenol (OPP) was too small to calculate. Both BHA and OPP induced a decrease in gene expression of ERα and an increase in that of progesterone receptor in a time-dependent manner. These effects were similar to that of 17β-estradiol, a though much higher concentrations were required for these compounds than 17β-estradiol. These results may suggest that we should be careful not to ingest excessive food additives.<br>

収録刊行物

  • 薬学雑誌

    薬学雑誌 123 (6), 443-452, 2003-06-01

    公益社団法人 日本薬学会

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