人工酵素によるゲノム編集工学とその応用，可能性

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  • 野村 渉
    東京医科歯科大学生体材料工学研究所

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タイトル別名
  • Application and Potential of Genome Engineering by Artificial Enzymes
  • Symposium Review 人工酵素によるゲノム編集工学とその応用,可能性
  • Symposium Review ジンコウ コウソ ニ ヨル ゲノム ヘンシュウ コウガク ト ソノ オウヨウ,カノウセイ

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  Artificial zinc finger proteins (ZFPs) consist of Cys2-His2-type modules composed of approximately 30 amino acids that adopt a ββα structure and coordinate a zinc ion. ZFPs recognizing specific DNA target sequences can substitute for the binding domains of various DNA-modifying enzymes to create designer nucleases, recombinases, and methylases with programmable sequence specificity. Enzymatic genome editing and modification can be applied to many fields of basic research and medicine. The recent development of new platforms using transcription activator-like effector (TALE) proteins or the clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas) system has expanded the range of possibilities for genome-editing technologies. These technologies empower investigators with the ability to efficiently knockout or regulate the functions of genes of interest. In this review, we discuss historical advancements in artificial ZFP applications and important issues that may influence the future of genome editing and engineering technologies. The development of artificial ZFPs has greatly increased the feasibility of manipulating endogenous gene functions through transcriptional control and gene modification. Advances in the ZFP, TALE, and CRISPR/Cas platforms have paved the way for the next generation of genome engineering approaches. Perspectives for the future of genome engineering are also discussed, including applications of targeting specific genomic alleles and studies in synthetic biology.<br>

収録刊行物

  • 薬学雑誌

    薬学雑誌 135 (3), 405-414, 2015-03-01

    公益社団法人 日本薬学会

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