Studies on the Enhancing Action of Isoproterenol to the Metabolism of Mitomycin C (Pharmacological)

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  • イソプロテレノールによるマイトマイシン C の代謝亢進作用(薬理学)
  • イソプロテレノールによるマイトマイシンCの代謝亢進作用
  • イソプロテレノール ニ ヨル マイトマイシン C ノ タイシャ コウシン サヨ

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Abstract

The influence of isoproterenol (IPN) on the metabolism of mitomycin C (MMC) was studied in rat ascites hepatoma AH130 cells. Exposure of the cells to IPN caused an elevation in the metabolic activity of MMC in the cell sonicates in the absence of the regenerating system of the reduced form of nicotinamide adenine dinucleotide phosphate (NADPH), though did not change the metabolism of MMC in the presence of the NADPH-regenerating system. Intracellular concentrations of NADPH decreased in proportion to the treatment time and the concentration of MMC, but the decrease of NADPH was inhibited by the combined treatment with IPN and MMC. IPN did not influence the activities of the metabolic enzymes of MMC and the NADPH-producing enzymes. IPN did not also change the intracellular amount of vinblastine which was taken up into the cells by passive transport similarly to MMC. From these results, our previous observations that IPN elevated the uptake of MMC into the cells were suggested to result from the enhancement of the metabolic activity but not that of the membrane transport of MMC. The enhancement of the metabolic activity seemed to be due to the increased potency of the cells to regenerate the intracellular NADPH consumed by the metabolism of MMC.

Journal

  • YAKUGAKU ZASSHI

    YAKUGAKU ZASSHI 106 (11), 1057-1063, 1986

    The Pharmaceutical Society of Japan

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