Determination of Scoporia Extract in Commercial Gastrointestinal Drugs by High-Performance Liquid Chromatography

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  • 高速液体クロマトグラフィーによる胃腸薬中のロートエキスの定量
  • コウソク エキタイ クロマトグラフィー ニ ヨル イチョウヤクチュウ ノ ロー

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Description

Tropane alkaloids, contained in gastrointestinal drugs including Scopolia Extract, were determined using high-performance liquid chromatographic method. Tropane alkaloids in commercial drugs were extracted with 0.5 N hydrochloric acid test solution. This solution was adjusted to pH 10.0 with a strong ammonia solution, extracted with ethyl ether, and separated on TSKgel ODS-120T (4.6mm i.d.×15cm ; temperature, 30°C ; flow rate, 1.5 ml/min ; detector, UV 210 nm) with a mixture of 1/15 M sodium phosphate buffer (pH 2.5) and acetonitril (68 : 32) containing 12 mM sodium dodecyl sulfate. The contents of atropine in the gastrointestinal drugs were 0.25-0.34 mg (coefficient of variation=0.20-2.61%) a day.

Journal

  • YAKUGAKU ZASSHI

    YAKUGAKU ZASSHI 112 (12), 944-949, 1992

    The Pharmaceutical Society of Japan

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