チトクロームb<SUB>5</SUB>を伴ったチトクロームP-450(17α-ヒドロキシラーゼ/リアーゼ)酸素添加酵素系の触媒するΔ<SUP>16</SUP>-C<SUB>19</SUB>-ステロイド合成活性

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タイトル別名
  • Δ<SUP>16</SUP>-C<SUB>19</SUB>-Steroid Synthesis Catalyzed by Cytochrome P-450 (17α-Hydroxylase/Lyase)-Linked Oxygenase System Involving Cytochrome b<SUB>5</SUB>
  • チトクロームb5を伴ったチトクロームP-450(17α-ヒドロキシラーゼ/リアーゼ)酸素添加酵素系の触媒するΔ16-C19-ステロイド合成活性
  • チトクローム b5 オ トモナッタ チトクローム P-450 17 アルファ
  • &Delta;<SUP>16</SUP>-C<SUB>19</SUB>-Steroid Synthesis Catalyzed by Cytochrome P-450 (17&alpha;-Hydroxylase/Lyase)-Linked Oxygenase System Involving Cytochrome b<SUB>5</SUB>

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In order to clarify the effect of cytochrome b5 on Δ16-C19-steroid synthesizing activity in the testicular microsomes of neonatal pig, the effects of antibody and pyridine nucleotide on cytochrome b5 were investigated. Furthermore, the relationship between Δ16-C19-steroid synthesizing activity and cytochrome b5 was studied by use of adrenal microsomes or a reconstituted enzyme system of cytochrome P-450 (17α-hydroxylase/lyase) purified from adrenal microsomes. With anti-cytochrome b5 IgG no remarkable change was observed in the 17α-hydroxylase/lyase activity of testicular microsomes. Δ16-C19-Steroid synthesizing activity was, however, strongly inhibited. In the testicular microsomes or the reconstituted enzyme system of cytochrome P-450 (17α-hydroxylase/lyase) isolated from testicular microsomes, synergetic effects of nicotinamide adenine dinucleotide phosphate and nicotinamide adenine dinucleotide against the Δ16-C-19-steroid synthesizing activity and 17α-hydroxylase/lyase activity was observed. A strong effect was detected on the Δ16-C19-steroid synthesizing activity, while this synergetic effect against 17α-hydroxylase/lyase activity was weak. Although in adrenal microsomes little Δ16-C19-steroid synthesizing activity was detected, this activity was restored by the addition of cytochrome b5 isolated from testicular microsomes. Similarly to microsomes, Δ16-C19-steroid activity was restored by the addition of cytochrome b5 to the reconstituted enzyme system of cytochrome P-450 (17-hydroxylase/lyase) isolated from adrenal microsomes.

収録刊行物

  • 薬学雑誌

    薬学雑誌 107 (2), 157-163, 1987

    公益社団法人 日本薬学会

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