Separation Analysis of Acidic Components in Human Urine by High-speed Liquid Chromatography

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  • 高速液体クロマトグラフ法による人尿中の酸性成分の分離定量
  • コウソク エキタイ クロマトグラフホウ ニヨル ジン ニョウチュウ ノ サンセ

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Description

High-speed liquid chromatography employing an ultraviolet photometric detector (254 nm) was applied to the separation determination of hippuric acid, p-hydroxyphenylacetic acid, toluoylglycines, p-hydroxybenzoic acid and benzoic acid in human urine. Reversed-phase partition chromatography was carried out on a Zorbax CN column with 0.1M HClO4 (pH 3.5)-MeOH (75 : 25, v/v) as the eluent system with a Shimadzu Du Pont LC 2 liquid chromatograph. This method obviates the necessity of samples for isolation or derivation of these acids before assay. The only pretreatment necessary is extraction of samples from human urine with ethyl acetate. The accuracy of this method was found to be less than ±1% and the limits of detection were shown.

Journal

  • YAKUGAKU ZASSHI

    YAKUGAKU ZASSHI 99 (3), 319-322, 1979

    The Pharmaceutical Society of Japan

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