Sample Preparation for Shotgun Proteomics by Using Phase Transfer Surfactants

DOI 2 Citations
  • Masuda Takeshi
    Department of Pharmacology, School of Medicine, University of Washington
  • Ishihama Yasushi
    Graduate School of Pharmaceutical Science, Department of Molecular & Cellular BioAnalysis, Kyoto University

Bibliographic Information

Other Title
  • 相間移動溶解剤を用いたショットガンプロテオミクス用試料調製法

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Description

<p>We have reported a phase transfer surfactants (PTSs)-aided sample preparation protocol for shotgun proteomics. The PTS protocol using deoxycholate and lauroylsarcosin increases the solubility of membrane proteins and enhances trypsin and lysyl endopeptidase activities. The PTSs become more hydrophobic at acidic pH. Based on this property, PTSs are removable from sample solutions by the liquid-liquid extraction method with ethyl acetate as organic solvent, while peptides are remained in the aqueous phase. Compared with protocols using a RapiGest or a filter–aided sample preparation protocol, the number of identified proteins and the recovery of peptides are significantly increased in the PTS protocol, suggesting that the PTSs protocol is more effective for the large-scale unbiased proteomics than these protocols.</p>

Journal

  • Proteome Letters

    Proteome Letters 1 (2), 95-100, 2016

    Japanese Proteomics Society

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Details 詳細情報について

  • CRID
    1390282681206262016
  • NII Article ID
    130006743378
  • DOI
    10.14889/jpros.1.2_95
  • ISSN
    24322776
  • Text Lang
    ja
  • Data Source
    • JaLC
    • CiNii Articles
    • Crossref
  • Abstract License Flag
    Disallowed

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