Alizarin Red S staining method for the effective detection of conodont elements in limestone

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  • Suzuki Kazuhiro
    Department of Earth and Environmental Sciences, Graduate School of Environmental Studies, Nagoya University Aichi Prefectural Meiwa High School
  • Mimura Koichi
    Department of Earth and Environmental Sciences, Graduate School of Environmental Studies, Nagoya University

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  • 石灰岩のコノドント検出を容易にするアリザリンレッドS染色法の開発
  • セッカイガン ノ コノドント ケンシュツ オ ヨウイ ニ スル アリザリンレッド S センショクホウ ノ カイハツ

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Conodont elements in limestone are generally collected from acid-insoluble residues by using heavy solutions (e.g., bromoform). To avoid the use of harmful heavy solutions, we have developed a new method of identifying conodont elements in limestone. Acid-insoluble residues were obtained by treating limestone samples with 10% acetic acid solution. The collected residues were then soaked in a 10% acetic acid solution containing 0.1 wt.% Alizarin Red S for 5 days. Apatite (conodont elements, pellet pieces, abiotic apatite minerals, etc.) in the residues was stained a color between orange and light red, enabling it to be easily distinguished from other minerals. This method enables conodont elements to be collected from limestone samples effectively and safely.

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