EFFECT OF TRYPSIN ON VIRAL SUSCEPTIBILITY OF VERO CELL CULTURES-CERCOPITHECUS KIDNEY LINE
-
- ITOH HEIHACHI
- <I>Toshiba Institute of Biological Science</I>
-
- MORIMOTO YOSHIO
- <I>Toshiba Institute of Biological Science</I>
-
- IWASE ISAO
- <I>Toshiba Institute of Biological Science</I>
-
- DOI YUTAKA
- <I>Toshiba Institute of Biological Science</I>
-
- SANPE TSUNEO
- <I>Toshiba Institute of Biological Science</I>
-
- NAKAJIMA MINORU
- <I>Toshiba Institute of Biological Science</I>
-
- OKAWA SHOICHIRO
- <I>Toshiba Institute of Biological Science</I>
-
- KATOH TOSHITADA
- <I>Toshiba Institute of Biological Science</I>
-
- ISHIKAWA MICHIO
- <I>Toshiba Institute of Biological Science</I>
-
- MURAMATSU SHINSUKE
- <I>Toshiba Institute of Biological Science</I>
Search this article
Description
Addition of dilute trypsin to the maintenance medium of Vero cell cultures made them far more susceptible to certain strains of parainfluenza type 1 (HVJ) and influenza A and B viruses. These findings are in line with those already reported on parainfluenza type 1 (HA-2) and type 4 viruses. To other virus strains belonging to myxo, picorna, reo, arbo, adeno, herpes, papova and poxvirus groups, the susceptibility was not enhanced by this manipulation. The difference of infectivity titers between those measured in Vero cell cultures under the trypsin-added condition (Vrt) and the ordinary ones (Vro) was taken as a criterion for the enhancement. In the serial passage tests of selected viruses in Vrt, most of viruses with the above titer-enhancement was proved transferable in the trypsin-added system, but remained nontransferable in Vro even after the passages in Vrt. Consequently, a possible emergence and selection of variants specially adapted to Vero cells is unlikely to occur in the Vrt system. Therefore, the present observations were considered on the basis of modified viral susceptibility of the cell culture.
Journal
-
- Japanese Journal of Medical Science and Biology
-
Japanese Journal of Medical Science and Biology 23 (4), 227-235, 1970
National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee
- Tweet
Details 詳細情報について
-
- CRID
- 1390282681216854528
-
- NII Article ID
- 130003778238
-
- NII Book ID
- AA1132885X
-
- COI
- 1:CAS:528:DyaE3MXhtV2gtb4%3D
-
- ISSN
- 18842828
- 00215112
-
- NDL BIB ID
- 8507092
-
- PubMed
- 4321639
-
- Text Lang
- en
-
- Data Source
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- Abstract License Flag
- Disallowed