CYTOTOXIC EFFECTS OF SCIRPENE COMPOUNDS, FUSARENON-X PRODUCED BY <I>FUSARIUM NIVALE</I>, DIHYDRONIVALENOL AND DIHYDROFUSARENON-X, ON HELA CELLS
-
- OHTSUBO KOHICHIRO
- <I>Department of Carcinogenesis and Cencer Susceptibility. The Institute of Medical Science, the University of Tokyo</I>
-
- SAITO MAMORU
- <I>Department of Carcinogenesis and Cencer Susceptibility. The Institute of Medical Science, the University of Tokyo</I>
Bibliographic Information
- Other Title
-
- Cytotoxic effects of scirpene compounds,fusarenon-X produced by Fusarium nivale,dihydronivalenol and dihydrofusarenon-X,HeLa cells
- CYTOTOXIC EFFECTS OF SCIRPENE COMPOUNDS, FUSARENON-X PRODUCED BY FUSARIUM NIVALE, DIHYDRONIVALENOL AND DIHYDROFUSARENON-X, ON HELA CELLS
Search this article
Description
Three toxic substances related to nivalenol were tested for their growth inhibiting activity and suppressive effects on DNA, RNA and protein syntheses of HeLa S3 cells in comparison with those of nivalenol.<BR>Fusarenon-X, isolated from culture filtrate of Fusarium nivale, showed a complete inhibition of growth of HeLa S3 cells at a concentration of 0.5 μg/ml. The ED50 for 3H-thymidine incorporation into DNA and 3H-leucine into protein were 0.1 and 0.13 μg/ml (2.8 and 3.6×10-7M), respectively, thus indicating about a 4 times potent activity as compared with that of nivalenol. No inhibi-tion of 3H-uridine incorporation into RNA occurred.<BR>Dihydronivalenol and dihydrof usarenon-X, -9-ene reduced substances of nivalenol and fusarenon-X, respectively, showed similar toxic effects on HeLa cells but with a six to ten times lower toxicity. The complete growth inhibition doses for the cells by both samples were around 3.0 μg/ml and the ED50 for 3H-thymidine and leucine incorporation were around 2.0 μg/ml. 3H-uridine incorporation was not remarkably inhibited.<BR>Biological effects of some other Fusaria metabolites having closely related chemical structures are discussed in association with nivalenol and f usarenon-X.
Journal
-
- Japanese Journal of Medical Science and Biology
-
Japanese Journal of Medical Science and Biology 23 (4), 217-225, 1970
National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee
- Tweet
Details 詳細情報について
-
- CRID
- 1390282681217174656
-
- NII Article ID
- 130003778237
-
- NII Book ID
- AA1132885X
-
- COI
- 1:STN:280:CS6D2sjjsFY%3D
-
- ISSN
- 18842828
- 00215112
-
- NDL BIB ID
- 8507091
-
- PubMed
- 5312796
-
- Text Lang
- en
-
- Data Source
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- Abstract License Flag
- Disallowed
