Isolation and Complete Genome Sequencing of Zika Virus Imported from the Dominican Republic to Japan

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  • Ozawa Hiroki
    Department of Microbiological Testing and Research, Yokohama City Institute of Public Health
  • Tajima Shigeru
    Department of Virology 1, National Institute of Infectious Diseases
  • Nakayama Eri
    Department of Virology 1, National Institute of Infectious Diseases
  • Kato Kengo
    Pathogen Genomics Center, National Institute of Infectious Diseases
  • Yamashita Akifumi
    Pathogen Genomics Center, National Institute of Infectious Diseases
  • Sekizuka Tsuyoshi
    Pathogen Genomics Center, National Institute of Infectious Diseases
  • Kuroda Makoto
    Pathogen Genomics Center, National Institute of Infectious Diseases
  • Usuku Shuzo
    Department of Microbiological Testing and Research, Yokohama City Institute of Public Health

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<p>Zika virus (ZIKV) infection has been documented within Central and South America, Asia, and Africa. Here we report the isolation of virus from a patient infected with ZIKV returning to Japan from the Dominican Republic. The ZIKV strain was imaged by electron microscopy and its complete genome sequence was analyzed. Phylogenetic analysis and molecular characterization revealed that the strain was of Asian lineage, and carried 2 unique mutations in its NS5 region. These mutations are characteristic of strains that originated in the Dominican Republic and the USA in 2016.</p>

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