Effect of Serum Components on Biofilm Formation by <i>Aspergillus fumigatus</i> and Other <i>Aspergillus</i> Species

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  • Wuren Tuya
    Division of Clinical Research, Medical Mycology Research Center, Chiba University
  • Toyotome Takahito
    Division of Clinical Research, Medical Mycology Research Center, Chiba University Research Unit for Risk Analysis, Research Center for Animal Hygiene and Food Safety, Obihiro University of Agriculture and Veterinary Medicine
  • Yamaguchi Masashi
    Division of Molecular Biology, Medical Mycology Research Center, Chiba University
  • Takahashi-Nakaguchi Azusa
    Division of Bio-resources, Medical Mycology Research Center, Chiba University
  • Muraosa Yasunori
    Division of Clinical Research, Medical Mycology Research Center, Chiba University
  • Yahiro Maki
    Division of Clinical Research, Medical Mycology Research Center, Chiba University
  • Wang Dan-Ni
    Division of Clinical Research, Medical Mycology Research Center, Chiba University
  • Watanabe Akira
    Division of Clinical Research, Medical Mycology Research Center, Chiba University Division of Control and Treatment of Infectious Diseases, Chiba University Hospital
  • Taguchi Hideaki
    Division of Clinical Research, Medical Mycology Research Center, Chiba University
  • Kamei Katsuhiko
    Division of Clinical Research, Medical Mycology Research Center, Chiba University Division of Control and Treatment of Infectious Diseases, Chiba University Hospital

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Other Title
  • Effect of Serum Components on Biofilm Formation by Aspergillus fumigatus and Other Aspergillus Species

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Abstract

Biofilm production by microorganisms is critical for their pathogenicity. Serum promotes biofilm production by Aspergillus fumigatus; however, its effects on other Aspergillus spp. have not been reported. We analyzed biofilm formation by five Aspergillus spp., i.e., A. fumigatus, A. flavus, A. nidulans, A. niger, and A. terreus, and examined the effects of serum/serum proteins such as fetal bovine serum (FBS), fetuin A, and bovine serum albumin (BSA) on hyphal growth, hyphal branching, and extracellular matrix (ECM) formation. The antifungal susceptibility of A. fumigatus isolates that formed biofilms was also examined. All serum/serum proteins promoted the growth of all these fungal species; growth promotion was most evident with FBS, followed by fetuin A and BSA. This effect was most evident in case of A. fumigatus and least evident in case of A. terreus. Electron microscopy showed thick ECM layers surrounding fungal cell walls after culture with FBS, particularly in A. fumigatus. An increase in hyphal branching caused by fetuin A was the highest in case of A. fumigatus and A. nidulans. Biofilm-forming A. fumigatus showed resistance to most antifungal agents, although a synergism of micafungin and amphotericin B was suggested. Our results indicate that serum promotes biofilm formation, including thick ECM, by many Aspergillus spp., particularly A. fumigatus, and that this may be closely related to its virulence.

Journal

  • Japanese Journal of Infectious Diseases

    Japanese Journal of Infectious Diseases 67 (3), 172-179, 2014

    National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee

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