Relationship between <i>stx</i> Genotype and Stx2 Expression Level in Shiga Toxin-Producing <i>Escherichia coli</i> O157 Strains
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- Kawano Kimiko
- Miyazaki Prefectural Institute for Public Health and Environment
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- Ono Hidetoshi
- Hyuga Meat Inspection Center
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- Iwashita Osamu
- Tsuno Meat Inspection Center
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- Kurogi Mai
- Tsuno Meat Inspection Center
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- Haga Takeshi
- Department of Microbiology, University of Miyazaki
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- Maeda Ken
- Department of Microbiology, Yamaguchi University
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- Goto Yoshitaka
- Department of Microbiology, University of Miyazaki
Bibliographic Information
- Other Title
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- Relationship between stx Genotype and Stx2 Expression Level in Shiga Toxin-Producing Escherichia coli O157 Strains
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Abstract
To determine the expression level of Shiga toxin (Stx) 2-related toxins (Stx2 and Stx2c) produced by each of 33 Stx-producing Escherichia coli (STEC) O157 strains, stx2 and stx2c mRNAs (stx2-related mRNA) were measured using real-time PCR with primers that recognize sequences common to stx2 and stx2c. The amount of Stx2 and Stx2c protein was measured using a reversed passive latex agglutination (RPLA) kit. Expression of stx2-related mRNA was significantly higher in STEC O157 strains carrying the stx2 gene (i.e., stx2, stx1/stx2, or stx2/stx2c) than in most strains that carried the stx2c gene but not the stx2 gene (i.e., stx2c or stx1/stx2c). RPLA might not measure the precise amount of each toxin variant; nevertheless, stx2-inclusive strains had 40-fold higher mean toxin titers than did strains that carried the stx2c gene but not the stx2 gene, with the exception of 1 stx2c strain. Interestingly, 1 stx2c strain that was isolated from a patient with severe hemorrhagic diarrhea had the highest stx2-related mRNA expression and the highest toxin titer of all 33 STEC O157 strains. Taken together, these findings indicated that measurement of stx2-related mRNA expression could reflect differences in production levels of toxins among STEC strains.
Journal
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- Japanese Journal of Infectious Diseases
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Japanese Journal of Infectious Diseases 65 (4), 322-325, 2012
National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee
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Details 詳細情報について
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- CRID
- 1390282681217995136
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- NII Article ID
- 130001931831
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- NII Book ID
- AA1132885X
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- ISSN
- 18842836
- 13446304
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- NDL BIB ID
- 023840639
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- PubMed
- 22814156
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed