New Validated Rapid Screening Methods for Identifying Kudoa septempunctata in Olive Flounder (Paralichthys olivaceus)
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- Sugita-Konishi Yoshiko
- Graduate School of Environmental Health Science, Azabu University
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- Fukuda Yutaka
- Fisheries Research Division, Oita Prefectural Agriculture, Forestry and Fisheries Research Center
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- Mori Koh-ichiro
- National Research Institute of Aquaculture
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- Mekata Toru
- National Research Institute of Aquaculture
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- Namba Toyohiko
- Incorporated Foundation Tokyo Kenbikyo-in
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- Kuroda Makoto
- Pathogen Genomics Center, National Institute of Infectious Diseases
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- Yamazaki Akiko
- Division of Microbiology, National Institute of Health Sciences
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- Ohnishi Takahiro
- Division of Microbiology, National Institute of Health Sciences
書誌事項
- タイトル別名
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- New Validated Rapid Screening Methods for Identifying <i>Kudoa septempunctata</i> in Olive Flounder (<i>Paralichthys olivaceus</i>)
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抄録
Kudoa septempunctata is a newly identified causative agent of foodborne diseases associated with consuming raw olive flounder. Qualitative PCR and quantitative real-time PCR have been used as notification methods to identify K. septempunctata in Japan. However, these methods require expensive equipment and are time-consuming (2–3 h for screening). To address these problems, in this study, we developed new rapid and simple methods using real-time loop-mediated isothermal amplification (LAMP) and nucleic acid sequence based amplification-nucleic acid chromatography (NASBA-NAC). Using these methods, the total procedure required approximately 45 min and did not require any expensive equipment. With regard to validating these new methods in comparison with the notification methods used in Japan, we performed an inter-laboratory study of 5 laboratories using samples that included olive flounders infected with 4 different amounts of K. septempunctata. These results demonstrated that the sensitivity of NASBA-NAC was equivalent to that of qualitative PCR, and that the sensitivity of real-time LAMP was equivalent to that of quantitative real-time PCR, which indicated that these new methods were acceptable screening methods for identifying K. septempunctata.
収録刊行物
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- Japanese Journal of Infectious Diseases
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Japanese Journal of Infectious Diseases 68 (2), 145-147, 2015
国立感染症研究所 Japanese Journal of Infectious Diseases 編集委員会
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詳細情報 詳細情報について
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- CRID
- 1390282681218541312
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- NII論文ID
- 130004757133
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- NII書誌ID
- AA1132885X
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- ISSN
- 18842836
- 13446304
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- NDL書誌ID
- 026281075
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- PubMed
- 25672358
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 使用不可