Transient expression of tryptophan oxygenase gene from Anopheles gambiae in the green eye mutant of the housefly, Musca domestica (Diptera: Muscadae).

  • Yoshiyama Mikio
    Laboratory of Applied Zoology, Institute of Agriculture and Forestry, University of Tsukuba
  • Honda Hiroshi
    Laboratory of Applied Zoology, Institute of Agriculture and Forestry, University of Tsukuba
  • Kimura Kiyoshi
    Laboratory of Apiculture, Department of Animal Genetics, National Institute of Animal Industry

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A plasmid construct containing the gene encoding tryptophan oxygenase (to) from Anopheles gambiae was introduced into green eye color mutants (ge) of the housefly, Musca domestica. Several variations in eye color were observed in 38.8% of the surviving G0 adults. The expression of A. gambiae to in the distantly related Diptera, housefly, implies conservation of the tryptophan oxygenase gene in the other Diptera and may be used for monitoring transformants as a marker in insects which have mutants homologous to Drosophila melanogaster vermilion.

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