Analysis of mariner-like elements in the smaller tea tortrix, Adoxophyes honmai and the summer fruit tortrix, Adoxophyes orana fasciata (Lepidoptera: Tortricidae).

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  • Yoshiyama Mikio
    Institute of Agriculture and Forestry, University of Tsukuba
  • Honda Hiroshi
    Institute of Agriculture and Forestry, University of Tsukuba
  • Noguchi Hiroshi
    Department of Environmental Biology, National Institute of Agro-Environmental Sciences
  • Kimura Kiyoshi
    Department of Animal Genetics, National Institute of Animal Industry

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Abstract

The mariner-like element (MLE) in two closely related leaf-roller moths, the smaller tea tortrix, Adoxophyes honmai and the summer fruit tortrix, Adoxophyes orana fasciata (Lepidoptera: Tortricidae) were surveyed. By a series of PCR and Southern blotting experiments, there were at least two types of MLEs in these two species. Sequence dissimilarity of each MLE suggests a possible polyphyletic origin and subsequent vertical inheritance within each species. In addition, a full-length MLE was isolated from a genomic library of A. honmai. This element, named Ahmarl, was 1,294 bp long with 27 bp inverted terminal repeats (ITRs). This element is apparently inactive due to a frameshift in the open reading frame (ORF) region, but a putative ORF could be observed by a single nucleotide removal, suggesting that the MLE in this species could have had the ability to transpose in the near past. Ahmarl may be suitable as a transformation vector with slight modification.

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