Antitumor effect of orally administered extracts from fruit body of Grifola frondosa (maitake).

Yamada Yuko, Nanba Hiroaki, Kuroda Hisatora

doi:10.11250/chemotherapy1953.38.790

We conducted a footpad swelling test to clarify whether a delayed type hypersensitivity (DTH) reaction against tumor antigen is potentiated by oral administration of D fraction extracted and purified from fruit body of Grifola frondosa (maitake), with a ratio of β-glucan to protein of 7: 3. When the antigen was injected in D fraction administered tumorbearing mice (D-mice), footpad swelling was observed, suggesting that a DTH reaction against the tumor antigen was potentiated by oral administration of D-fraction. Winn assay was carried out with whole spleen cells from D-mice and Lyt-2^-spleen cells in which Lyt-2⁺ cells corresponding to cytotoxic T cells (CTL) were eliminated by treating with anti-Lyt-2 monoclonal antibody and complement. Even after CTL were eliminated, tumor neutralizing activity was at no time impaired, indicating that spleen cells other than CTL play a leading role in tumor cytotoxicity.<BR>We hypothesize from the above results, that a DTH reaction, manifested by the combined action of delayed hypersensitivity T cells (Tdh) and macrophages (MΦ), was potentiated by oral administration of D-fraction. Even when CTL are absent, there is a high degree of tumor involution. But D-fraction did not activate gut-associated lymphoid tissue (GALT) and Peyer's patch (PP), by oral administration.

Antitumor effect of orally administered extracts from fruit body of Grifola frondosa (maitake).

Bibliographic Information

Abstract

Journal

Keywords

Details 詳細情報について

Export

Report a problem

Antitumor effect of orally administered extracts from fruit body of Grifola frondosa (maitake).

Bibliographic Information

Abstract

Journal

Keywords

Details 詳細情報について

Export

Report a problem

Project list