併用効果測定のための２倍希釈系列を用いたｃｈｅｃｋｅｒｂｏａｒｄ法における問題点
書誌事項
- タイトル別名
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- Problems in the checkerboard method using serial twofold dilutions.
説明
We measured in vitro the effect of combined therapy against methicillin-resistant Staphylococcus aureus (MRSA) using the modified checkerboard method with serial 1.25-fold dilutions (1.25-fold method) and a conventional method using serial twofold dilutions (twofold method) to compare the results. Vancomycin and minocyclin, which have a low MIC value, and cefotiam, which has a high MIC value, were used. In the determination of MIC value, discrepancies were found between the twofold and 1.25-fold methods even in the same strain. Discrepancies between these two methods were also observed in the combination effect in some cases. In the twofold method, decreased intensity of the MIC value in combination could be detected only in the form of (1/2) n. Therefore, in this method results with a 1/2 decrease were outwardly obtained even with a decrease of less than 1/2. On the other hand, in the 1.25-fold method it could be detected in the form of (4/5) n, and no discrepancies were found between the results of this method and the expected decrease. Thus more exact FIC indexes and MIC values in combination were obtained. In addition, the present method could detect weak antagonism that could not be detected by the twofold method. We concluded that 1.25-fold serial dilution was better than twofold dilution in the assesment of combinations of an antibiotic with a low MIC value and one with a high MIC value.
収録刊行物
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- ＣＨＥＭＯＴＨＥＲＡＰＹ
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ＣＨＥＭＯＴＨＥＲＡＰＹ 42 (7), 841-848, 1994
公益社団法人 日本化学療法学会
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詳細情報 詳細情報について
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- CRID
- 1390282681255978368
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- NII論文ID
- 130004198547
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- ISSN
- 18845894
- 00093165
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可
