EVALUATION OF POTENTIAL NEPHROTOXICITY OF ANTIBIOTICS. I.

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  • UMEDA TAKASHI
    Department of Urology, Faculty of Medicine, The University of Tokyo
  • KOISO KENKICHI
    Department of Urology, Faculty of Medicine, The University of Tokyo

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Other Title
  • 抗生物質の腎毒性に関する研究 I
  • コウセイ ブッシツ ノ ジン ドクセイ ニカンスルケンキュウ 1 シロネズミ
  • Effects on Protein Synthesis and Stability of Lysosome of Rat Kidney
  • シロネズミ腎の蛋白代謝およびリソゾーム膜に及ぼす影響を中心に

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Abstract

In order to assess the potential nephrotoxicity exerted by various antibiotics with therapeutic dosis, effects of antibiotics administered into Wistar rat in vitro and in vivo were investigated in terms of protein synthesis, nucleic acid metablism and stability of lysosome.<BR>Kanamycin, streptomycin, gentamicin, colistin as well as chloramphenicol inhibited the incorporation of orotic acid-6-14C into rat renal nuclear RNA.<BR>The incorporation of orotic acid-6-14C into RNA of the free polysome fraction of the rat kidney slices was inhibited by kanamycin, streptomycin, colistin and tetracycline.<BR>The incorporation of L-leucine-14C into the free polysome fraction was demonstrated to be reduced by streptomycin, chloramphenicol and tetracycline administered in vivo.<BR>The incorporation of orotic acid-6-14C into RNA of the bound polysome fraction of rat kidney slices was reduced by kanamycin, streptomycin, chloramphenicol and cephalothin. The incorporation of L-leucine-14C into the bound polysomes fromes fraction was inhibited by streptomycin and colistin.<BR>Marked reduction of the incorporation of L-leucine-14C into the free polysome fraction was noticed by kanamycin, streptomycin, gentamicin and chloramphenicol added directly to cell-free protein synthesis system. Also, marked reduction of the incorporation of L-leucine-14C into the bound polysome fraction was manifested by kanamycin, streptomycin, gentamicin and colistin.<BR>Stability of lysosome was disturbed by either kanamycin or streptomycin.<BR>Clinical implication of these nephrotoxicity of antibiotics was discussed.

Journal

  • CHEMOTHERAPY

    CHEMOTHERAPY 25 (10), 2978-2983, 1977

    Japanese Society of Chemotherapy

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