EXPERIMENTAL CHEMOTHERAPY AGAINST <I>SERRATIA MARCESCENS</I>. III

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Other Title
  • <I>Serratia marcescens</I>に関する実験的化学療法第3報
  • Serratio marcescens ニ カンスル ジッケンテキ カガク リ
  • Antibacterial Picture of Aminoglycoside Antibiotics
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Description

Employing phase contrast microscope, scanning and transmission electron microscopes, antibacterial picture of aminoglycoside antibiotics such as gentamicin (GM) and dibekacin (DKB) for Serratia marcescens T-55 was morphologically studied.<BR>By phase contrast microscope, the growth of bacteria was observed at 1/8 and 1/4 × MIC of DKB and GM, almost same as the control. At 1/2 × MIC of DKB and GM, the growth of bacteria was completely inhibited. At 1, 5 and 10×MIC, a hole-like structure was observed in the cytoplasm of bacterial cell.<BR>By scanning electron microscope, a markedly collapsed structure was observed on the surface layer of bacterial cell in 1, 5 and 10 × MIC of DKB and GM, and the finding, which could be considered to correspond to the hole-like structure noted by phase contrast microscope, were also observed. Observation with transmission electron microscope revealed the presence of a layer (capsule-like structure) in the outer layer of cell wall in the control. Marked plasmolysis and a thinning of cytoplasm in bacterial cell were noted one hour after the exposure of 1×MIC of DKB and GM, and also almost the same phenomenon was found by the exposure of 5×MIC of DKB and GM.<BR>In the study of the antibacterial effect of aminoglycoside antibiotics against E. coli, a bleb formation was observed, and against Ps. aeruginosa, a cutted face was found in the outer layer of cell wall, but in Serratia those changes were not noted. Thus, aminoglycoside antibiotics appear to have a differential antibacterial action against each organism.

Journal

  • CHEMOTHERAPY

    CHEMOTHERAPY 25 (2), 372-379, 1977

    Japanese Society of Chemotherapy

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