書誌事項
- タイトル別名
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- Determination of ritipenem in human plasma and urine by high performance liquid chromatography
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説明
A high performance liquid chromatographic method was developed for determination of ritipenem (RIPM), the active metabolite of ritipenem acoxil (RIPM-AC){(+)-(5R, 6S)-acetoxymethyl 3-carbamoyloxymethyl-6-[(1R)-1-hydroxyethyl]-7-oxo-4-thia-1-azabicyclo [3.2.0] hept-2-ene-2-carboxylate monohydrate}, in human plasma and urine. Plasma samples were deproteinized by the ultrafiltration method, and the filtrates were analyzed by a reversed-phase column using the two-sided bracketing injection technique. In the plasma samples spiked with RIPM, the calibration curve showed good linearity in the range of 0-5.0 μg/ml. The recovery of RIPM was quantifiable; coefficients of within-day and between-day variation were less than 4.8% and 3.2%, respectively. In the urine samples spiked with RIPM, the calibration curve in the range of 0-1000μg/ml and the reproducibilities were similarly satisfactory. The determination limits were 0.05μg/ml in plasma and 2.5μg/ml in urine. The stability of RIPM in plasma was confirmed at -20°C for a month in addition to stabilizer. Correlations between the proposed method and bioassay were satisfactory for both plasma and urine samples.
収録刊行物
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- 日本化学療法学会雑誌
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日本化学療法学会雑誌 43 (Supplement3), 91-96, 1995
公益社団法人 日本化学療法学会
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詳細情報 詳細情報について
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- CRID
- 1390282681262999040
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- NII論文ID
- 10005467344
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- NII書誌ID
- AN10472127
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- ISSN
- 18845886
- 13407007
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可
