Enhanced Saccharification by Trichoderma reesei Expressing a P-Glucosidase from Magnaporthe oryzae

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  • Takahashi Machiko
    Iwate Biotechnology Research Center Iwate Biotechnology Research Center
  • Nakajima Masahiro
    Iwate Biotechnology Research Center Iwate Biotechnology Research Center
  • Nakano Yuki
    Iwate Biotechnology Research Center Iwate Biotechnology Research Center
  • Takeda Takumi
    Iwate Biotechnology Research Center Iwate Biotechnology Research Center

書誌事項

タイトル別名
  • Enhanced Saccharification by <I>Trichoderma reesei </I>Expressing a β-Glucosidase from <I>Magnaporthe oryzae</I>
  • Enhanced Saccharification by Trichoderma reesei Expressing a ^|^beta;-Glucosidase from Magnaporthe oryzae

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抄録

A his7-tagged β-glucosidase from Magnaporthe oryzae (MoCel3A) was expressed under the control of a cellobiohydrolase I (cbh1) promoter in Trichoderma reesei. Accumulation of extracellular his7-tagged MoCel3A and glucose production were evaluated during growth on a variety of substrates. Enzyme preparations obtained from the recombinant T. reesei strain grown in the presence of cellulose produced more his7-tagged MoCel3A, exhibited greater hydrolytic activity towards pNPG and produced more glucose from PSC, cellulose, rice straw and laminarin than the wild type parent. These results indicate that expression of his7-tagged MoCel3A enhanced glucose production not only from β-1,4-glucan but also from β-1,3-glucan. More his7-tagged MoCel3A accumulated when the recombinant strain was grown in the presence of cellooligosaccharides with various degrees of polymerization. Interestingly, accumulation of his7-tagged MoCel3A was highly enhanced by growth on laminarioligosaccharides. An active enzyme preparation was obtained from the recombinant strain grown on rice straw, an inexpensive and renewable substrate.

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