蛋白工学による好アルカリ性Bacillus由来のカルシウムを含まないα-アミラーゼの耐熱化

DOI 日本農学文献記事索引 Web Site Web Site 被引用文献4件 参考文献28件

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  • Improvement of Thermostability of a Calcium-free .ALPHA.-Amylase from an Alkaliphilic Bacillus sp. by Protein Engineering.
  • Improvement of Thermostability of a Calcium-free α-Amylase from an Alkaliphilic Bacillus sp. by Protein Engineering
  • Improvement of Thermostability of a Calcium free アルファ Amylase from an Alkaliphilic Bacillus sp by Protein Engineering

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A novel α-amylase (AmyK38) from an alkaliphilic Bacillus designated KSM-K38 is strongly resistant to chelators and oxidative reagents and contains no calcium. However, thermostabilization of AmyK38 is essential if it is to have industrial applications. Several chimeric enzymes between AmyK38 and the thermostable Arg181-G1y182-deleted mutant (dRG) of an α-amylase AmyK were constructed. A chimeric enzyme containing the N-terminal 21 amino acid residues of dRG was found to have higher thermostability than the parental AmyK38. By site-directed mutagenesis, AmyK38 was successfully thermostabilized by the single substitution of Tyrl1 by Phe without any changes in the kinetic features.

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