Arthrobacter sp.K-1株由来のβ-フルクトフラノシダーゼ遺伝子のクローニングと大腸菌における発現
書誌事項
- タイトル別名
-
- Cloning and Expression of .BETA.-Fructofuranosidase Gene from Arthrobacter sp. K-1.
- Cloning and Expression of β-Fructofuranosidase Gene from Arthrobacter sp. K-1
- Cloning and Expression of ベータ Fructofuranosidase Gene from Arthrobacter sp K 1
この論文をさがす
抄録
A gene encoding an extracellular β-fructofuranosidase (β-FFase), designated bff A, was clonedfrom the chromosomal DNA of Arthrobacter sp. K-1, and expressed in Escherichia coli. The putativebff A consisted of 1734 by encoding β-FFase of 578 amino acids. Comparison of the DNA sequencedata with the N-terminal amino acid sequence of the purified enzyme indicated the presenceof a precursor protein with a signal sequence of 36 residues. The deduced amino acid sequence ofthe β-FFase demonstrated a high degree of homology with that of levansucrase from Acetobacterdiazotrophicus. The N-terminal amino acid sequence and some enzymatic properties of the recombinantenzyme were identical to those of the native enzyme.
収録刊行物
-
- Ｊｏｕｒｎａｌ ｏｆ Ａｐｐｌｉｅｄ Ｇｌｙｃｏｓｃｉｅｎｃｅ
-
Ｊｏｕｒｎａｌ ｏｆ Ａｐｐｌｉｅｄ Ｇｌｙｃｏｓｃｉｅｎｃｅ 49 (3), 291-296, 2002
日本応用糖質科学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390282681268727424
-
- NII論文ID
- 10009257130
-
- NII書誌ID
- AN10453916
-
- ISSN
- 13403494
- 18807291
- 13447882
- http://id.crossref.org/issn/13447882
-
- COI
- 1:CAS:528:DC%2BD38Xms1Smt7w%3D
-
- NDL書誌ID
- 6254335
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可
