Casein kinase in cytosol of rat and mouse mammary epithelial cells isolated from histone kinase by MgCl2 treatment and influence of pregnancy and lactation on the enzyme activity.

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  • ASAGA HISATERU
    Department of Biochemistry, School of Medicine, Kyorin University
  • MUTO KINUKO
    Department of Biochemistry, School of Medicine, Kyorin University
  • HOMAREDA HIDEKO
    Department of Biochemistry, School of Medicine, Kyorin University
  • KOMINE SENICHI
    Department of Biochemistry, School of Medicine, Kyorin University

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  • Casein Kinase in Cytosol of Rat and Mou

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Rat mammary glands contain cyclic AMP-independent casein kinase and cyclic AMP-dependent histone kinase. The former was easily isolated from cyclic AMPdependent histone kinase by MgCl2 treatment. Mammary casein kinase was not activated by cyclic nucleotides, and Mg++ and ATP were required for activation.<BR>The specific activity of casein kinase in cytosol of rat mammary epithelial cells increased 2 to 3-fold during pregnancy and lactation.<BR>Cytosol of mouse mammary epithelial cells also contained cyclic AMP-independent casein kinase, and the activity of this enzyme was about three times that of the Golgi fraction.

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