レチノイン酸刺激によるラット上頚部交感神経節内組織トランスグルタミナーゼの誘導

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  • Induction of Tissue Transglutaminase in Rat Superior Cervical Sympathetic Ganglia in Response to Retinoic Acid.
  • レチノインサン シゲキ ニヨル ラットジョウ ケイブ コウカン シンケイセツナ

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The addition of retinoic acid (RA, 2×10-6M) to physiological saline solution markedly increased the activity of Ca2+ -dependent tissue transgiutaminase (TGase) (ca. 3.1-fold), which stabilizes newly formed protein assemblies at the synapse, in isolated rat superior cervical sympathetic ganglia, which are rich in synapses, fallowing in vitro aerobic incubation for 3h at 37°C. The RA-induced enhancement of ganglionic TGase activity was completely eliminated in the presence of either actinomycin D (1.0μg/ml), a depressor of transcriptional activity, or cycloheximide (10μg/ml), a potent inhibitor of protein synthesis. Kinetic studies showed that the stimulation of ganglionic TGase activity evoked by RA addition was associated with only an increase in the Vmax value. Thus, the enzyme protein of TGase might be synthesized de novo in the ganglia in response to RA. These findings imply that RA also participates in phenomena involving synaptic activity such as memory.

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