Isolation and Characterization of Arabinogalactan-protein from the Fruit of Lycium chinense Mill.

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  • Qin Xiaoming
    The United Graduate School of Agricultural Science, Gifu University, Science of Biological Resources
  • Yamauchi Ryo
    The United Graduate School of Agricultural Science, Gifu University, Science of Biological Resources
  • Aizawa Koichi
    Research Institute, Kagome Co., Ltd.
  • Inakuma Takahiro
    Research Institute, Kagome Co., Ltd.
  • Kato Koji
    The United Graduate School of Agricultural Science, Gifu University, Science of Biological Resources

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  • クコ果実のアラビノガラクタン-プロテインについて

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Abstract

Two kinds of arabinogalactan-protein (Cp-1-C and -D) were obtained from the fruit of Lycium chinense Mill. In Cp-1-C, the carbohydrate was composed of arabinose and galactose at a ratio of 3:1, and O -glycosidically linked to both the serine and threonine residues of protein. Its molecular weight was 42, 000. In Cp-1-D, the ratio of arabinose to galactose was 1:1, and the O -glycosidical junction between the carbohydrate and protein was composed of serine residue. The molecular weight was 23, 000. From the results of structural characterization, both Cp-1-C and -D were assigned to type II arabinogalactan-protein (AGP), where a (1→6) linkage of galactose occurred mainly at the branching point in the case of Cp-1-C and mainly at the linear part in Cp-1-D.

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