Aqueous Two-phase Extraction System Using an Assembled Dextran-conjugated Magnetite Prepared by the Dextransucrase Reaction

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Dextransucrase reacts with sucrose to produce dextran and fructose. Dextransucrase is known to form a complex with the dextran product at the active site. Using this characteristic, dextran was introduced to the surface of magnetite through the dextransucrase reaction by reaction with sucrose to obtain dextran-conjugated magnetite. This modified magnetite was applied in an aqueous two-phase system for protein purification with the aid of an applied magnetic field. Two sizes of the magnetite used were 320 nm and 5.0 μm. Dextran-conjugated magnetite (320 nm), assembled magnetically, was able to rapidly extract hemoglobin with a high yield because the dextran on the surface of the magnetite has an entangled structure with a high density for capturing hemoglobin.

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