Thermoanaerobium brockii由来耐熱性トレハロースホスホリラーゼの精製とその諸性質
書誌事項
- タイトル別名
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- Purification and Characterization of Thermostable Trehalose Phosphorylase from Thermoanaerobium brockii.
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抄録
A thermostable trehalose phosphorylase (EC 2.4.1.64) from a thermophilic anaerobe, Thermoanaero bium brockii ATCC 35047, was purified to an electrophoretically homogeneous state by successive column chromatographies on DEAE-Toyopearl 6505, Butyl-Toyopearl 650 M and Ultrogel AcA 44 . The molecular weight of the enzyme was estimated to be 190, 000 Da by gel filtration, and 88, 000 Da by SDS-polyacrylamide gel electrophoresis. The enzyme showed the highest activity in the range of pH 7.0 to 7.5 for phosphorolysis, and pH 6.0 to 7.0 for synthesis . The optimum temperature of the enzyme was 70°C in both directions. The enzyme was stable from pH 6.0 to 9.0, and up to 60°C . This result showed that our trehalose phosphorylase was the most thermostable in those reported to date . Activity was inhibited by Cue, Hg2+, Mg2+, Mn2+, Pb2+, and Zn2+ The Kms for trehalose, Pi, D-glucose and R-D-glucose 1-phosphate were 0.97, 0.57, 2.4 and 0.75 mM, respectively.
収録刊行物
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- Journal of Applied Glycoscience
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Journal of Applied Glycoscience 46 (4), 399-405, 1999
日本応用糖質科学会
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詳細情報 詳細情報について
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- CRID
- 1390282681269596160
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- NII論文ID
- 10008259332
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- NII書誌ID
- AN10453916
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- ISSN
- 13403494
- 18807291
- 13447882
- http://id.crossref.org/issn/13447882
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- COI
- 1:CAS:528:DC%2BD3cXhtFCnsLs%3D
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- NDL書誌ID
- 4976502
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可