Oxidation of Uronic Acids by a Large Excess of Glucose Oxidase Preparations.
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- Kobayashi Mikihiko
- National Food Research Institute
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- Nishihara Hirofumi
- Department of Applied Bioresource Science, School of Agriculture, Ibaraki University
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- Kobayashi Shoichi
- National Food Research Institute
Bibliographic Information
- Other Title
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- 高濃度のグルコースオキシダーゼにとよるウロン酸の酸化
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Abstract
Three commercial enzyme preparations of glucose oxidase (GOD) showed an oxidizing ability of glucuronic acid and galacturonic acid to form sugar acids. The optimum conditions of GOD reaction were at pH 8.0 and 50°C, whereass those of uronic acid oxidase (UOD) reaction were at pH 3.5 and 40°C. In spite of extensive attempts to separate GOD from UOD, the isolation of each activity was unsuccessful, and UOD reaction resulted from the wide substrate specificity of GOD reaction. More than 6-fold higher Km values for glucuronic acid and galacturonic acid than for glucose might suggest that UOD reaction was the alternative reaction of GOD enzyme. However, a more definite conclusion on UOD activity should be drawn from further studies. The reaction products from uronic acids were analyzed by HPLC and paper chromatography, and some products were shown to be identical with sugar acids.
Journal
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- Journal of Applied Glycoscience
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Journal of Applied Glycoscience 46 (1), 1-7, 1999
The Japanese Society of Applied Glycoscience
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Details 詳細情報について
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- CRID
- 1390282681270090880
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- NII Article ID
- 10008258526
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- NII Book ID
- AN10453916
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- ISSN
- 13403494
- 18807291
- 13447882
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- DOI
- 10.5458/jag.46.1
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- NDL BIB ID
- 4785602
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- Text Lang
- en
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed