<b><i>Aspergillus niger</i> 由来組換え α-グルコシダーゼの<i>Emericella nidulans</i> での過剰発現,精製および諸性質</b>
書誌事項
- タイトル別名
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- Recombinant .ALPHA.-Glucosidase from Aspergillus niger. Overexpression by Emericella nidulans, Purification and Characterization
- Recombinant α-Glucosidase from Aspergillus niger. Overexpression by Emericella nidulans, Purification and Characterization
- Recombinant アルファ Glucosidase from Aspergillus niger Overexpression by Emericella nidulans Purification and Characterization
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抄録
An expression plasmid containing the aglA gene encoding Aspergillus niger GN-3 α-glucosidase was constructed and inserted into Emericella nidulans JCM10259. The transformant secreted about 61 mg/L of the recombinant α-glucosidase into its culture medium. The recombinant enzyme was purified from the culture filtrate through ammonium sulfate precipitation and three chromatographic steps. It was confirmed that, like wild-type A. niger GN-3 α-glucosidase, the purified recombinant enzyme consisted of two subunits. Although the molecular mass of the recombinant enzyme was slightly smaller than that of wild-type A. niger α-glucosidase (attributed to differences in glycosylation), the pH optima and substrate specificities of the wild-type and recombinant enzymes were comparable.
収録刊行物
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- Journal of Applied Glycoscience
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Journal of Applied Glycoscience 53 (1), 13-16, 2006
日本応用糖質科学会
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詳細情報 詳細情報について
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- CRID
- 1390282681270426368
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- NII論文ID
- 10016738494
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- NII書誌ID
- AN10453916
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- COI
- 1:CAS:528:DC%2BD28XislOmsL4%3D
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- ISSN
- 18807291
- 13447882
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- NDL書誌ID
- 7860000
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可