Recombinant .ALPHA.-Glucosidase from Aspergillus niger. Overexpression by Emericella nidulans, Purification and Characterization
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- Ogawa Masahiro
- Department of Biological Chemistry, College of Bioresource Sciences, Nihon University
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- Nishio Toshiyuki
- Department of Biological Chemistry, College of Bioresource Sciences, Nihon University
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- Minoura Kayo
- Department of Biological Chemistry, College of Bioresource Sciences, Nihon University
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- Uozumi Takeshi
- Department of Life Sciences, School of Agriculture, Meiji Univercity
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- Wada Masato
- Department of Biological Chemistry, College of Bioresource Sciences, Nihon University
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- Hashimoto Noriko
- Department of Biological Chemistry, College of Bioresource Sciences, Nihon University
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- Kawachi Ryu
- Department of Biological Chemistry, College of Bioresource Sciences, Nihon University
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- Oku Tadatake
- Department of Biological Chemistry, College of Bioresource Sciences, Nihon University
Bibliographic Information
- Other Title
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- <b><i>Aspergillus niger</i> 由来組換え α-グルコシダーゼの<i>Emericella nidulans</i> での過剰発現,精製および諸性質</b>
- Recombinant α-Glucosidase from Aspergillus niger. Overexpression by Emericella nidulans, Purification and Characterization
- Recombinant アルファ Glucosidase from Aspergillus niger Overexpression by Emericella nidulans Purification and Characterization
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Abstract
An expression plasmid containing the aglA gene encoding Aspergillus niger GN-3 α-glucosidase was constructed and inserted into Emericella nidulans JCM10259. The transformant secreted about 61 mg/L of the recombinant α-glucosidase into its culture medium. The recombinant enzyme was purified from the culture filtrate through ammonium sulfate precipitation and three chromatographic steps. It was confirmed that, like wild-type A. niger GN-3 α-glucosidase, the purified recombinant enzyme consisted of two subunits. Although the molecular mass of the recombinant enzyme was slightly smaller than that of wild-type A. niger α-glucosidase (attributed to differences in glycosylation), the pH optima and substrate specificities of the wild-type and recombinant enzymes were comparable.
Journal
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- Journal of Applied Glycoscience
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Journal of Applied Glycoscience 53 (1), 13-16, 2006
The Japanese Society of Applied Glycoscience
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Keywords
Details 詳細情報について
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- CRID
- 1390282681270426368
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- NII Article ID
- 10016738494
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- NII Book ID
- AN10453916
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- COI
- 1:CAS:528:DC%2BD28XislOmsL4%3D
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- ISSN
- 18807291
- 13447882
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- NDL BIB ID
- 7860000
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed