Developing and Engineering Enzymes for Manufacturing Amylose
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- Yanase Michiyo
- Biochemical Research Laboratory, Ezaki Glico Co., Ltd.
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- Takaha Takeshi
- Biochemical Research Laboratory, Ezaki Glico Co., Ltd.
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- Kuriki Takashi
- Biochemical Research Laboratory, Ezaki Glico Co., Ltd.
Bibliographic Information
- Other Title
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- アミロース製造に利用する酵素の開発と改良
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Description
Amylose is a functional biomaterial and is expected to be used for various industries. However at present, manufacturing of amylose is not done, since the purification of amylose from starch is very difficult. It has been known that amylose can be produced in vitro by using α-glucan phosphorylases. In order to obtain α-glucan phosphorylase suitable for manufacturing amylose, we isolated an α-glucan phosphorylase gene from Thermus aquaticus and expressed it in Escherichia coli. We also obtained thermostable α-glucan phosphorylase by introducing amino acid replacement onto potato enzyme. α-Glucan phosphorylase is suitable for the synthesis of amylose; the only problem is that it requires an expensive substrate, glucose 1-phosphate. We have avoided this problem by using α-glucan phosphorylase either with sucrose phosphorylase or cellobiose phosphorylase, where inexpensive raw material, sucrose or cellobiose, can be used instead. In these combined enzymatic systems, α-glucan phosphorylase is a key enzyme. This paper summarizes our work on engineering practical α-glucan phosphorylase for industrial processes and its use in the enzymatic synthesis of essentially linear amylose and other glucose polymers.
Journal
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- Journal of Applied Glycoscience
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Journal of Applied Glycoscience 54 (2), 125-131, 2007
The Japanese Society of Applied Glycoscience
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Details 詳細情報について
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- CRID
- 1390282681270438016
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- NII Article ID
- 10018617611
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- NII Book ID
- AN10453916
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- ISSN
- 18807291
- 13447882
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- NDL BIB ID
- 8828027
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed