Screening of Lactobionic Acid Producing Microorganisms.
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- Murakami Hiromi
- Osaka Municipal Technical Research Institute
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- Kawano Jyunko
- Faculty of Agriculture, Kinki University
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- Yoshizumi Hajime
- Faculty of Agriculture, Kinki University
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- Nakano Hirofumi
- Osaka Municipal Technical Research Institute
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- Kitahata Sumio
- Osaka Municipal Technical Research Institute
Bibliographic Information
- Other Title
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- ラクトビオン酸生産微生物の検索
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Abstract
Lactobionic acid (LA) is derived from lactose and expected to be a versatile material for grow-ing bifidobacterium and forming mineral salts with high solubility in water for supplements. We aimed to develop microbial or enzymatic production systems of LA. To this aim, we screened lactose-oxidizing microorganisms, and obtained a strain of Burkholderia cepacia. The lactose-oxidizing activity existed in the membrane fraction of disrupted cell preparation of the strain. Only oxygen was necessary for lactose-oxidizing activity as a proton acceptor. A crude cell-free enzyme preparation was prepared, and its oxidizing ability and other properties on several saccharides were examined. The cell-free preparation oxidized D-glucose, D-mannose, D-galactose, D-xylose, L-arabinose and D-ribose. It also reacted with lactose, cellobiose, maltose, maltotriose, maltotetaose and maltopentaose. The strain accumulated LA in the culture supernatant with no loss of lactose. The strain is advantageous to production of LA by both fermentation and enzymatic reaction.
Journal
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- Journal of Applied Glycoscience
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Journal of Applied Glycoscience 49 (4), 469-477, 2002
The Japanese Society of Applied Glycoscience
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Details 詳細情報について
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- CRID
- 1390282681270632448
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- NII Article ID
- 10009930172
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- NII Book ID
- AN10453916
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- ISSN
- 13403494
- 18807291
- 13447882
- http://id.crossref.org/issn/13447882
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- COI
- 1:CAS:528:DC%2BD38XotF2mt7s%3D
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- NDL BIB ID
- 6335489
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- Text Lang
- en
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed