Changes in distribution of molecular weight forms of biologically active and immunoreactive adrenocorticotropic hormone after adrenalectomy in rat anterior pituitary.
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- ITOH YOSHIHARU
- The Second Department of Internal Medicine, Osaka University Medical School
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- IIDA SAYOMI
- The Second Department of Internal Medicine, Osaka University Medical School
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- MORIWAKI KANAME
- The Second Department of Internal Medicine, Osaka University Medical School
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- TARUI SEIICHIRO
- The Second Department of Internal Medicine, Osaka University Medical School
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- KAWAKAMI FUSAO
- Department of Internal Medicine, Itami Municipal Hospital
書誌事項
- タイトル別名
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- Changes in Distribution of Molecular We
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説明
The concentration of ACTH in extracts of rat anterior pituitary was measured by both radioimmunoassay and bioassay at different stages following adrenalectomy. Both types of ACTH activity decreased the day immediately following adrenalectomy but increased gradually afterwards. Immunological ACTH activity increased to 250% of the control value and biological ACTH activity increased to 490% of control value 3 weeks after adrenalectomy. The increase in biological ACTH activity occurred earlier, and the rate of increase was greater, than that of the immunological ACTH activity.<BR>The distributions of molecular weight forms of ACTH in extracts of anterior pituitary lobes was determined by gel filtration. Three molecular weight forms of immunoassayable ACTH were detected. Biological ACTH activity appeared in the 2nd and the 3rd peaks. A striking change was observed after adrenalectomy in the distribution of biologically active forms of ACTH. The ratio of biological ACTH activity to immunological ACTH activity in each peak changed at various stages after adrenalectomy. This indicated the heterogenous nature of the ACTH included in eachpeak. At 2 and again at 3 weeks, biological activity markedly increased until it exceeded the immunological ACTH activity in the 2nd peak. Dexamethasone had little influence on the elution profile of either immunoassayable and biologically active ACTH in gel filtration. Adrenalectomy may possibly have an effect on the intracellular posttranslational processing of ACTH precursors which leads to the deveploment of biological ACTH activity.
収録刊行物
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- Endocrinologia Japonica
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Endocrinologia Japonica 28 (5), 685-695, 1981
一般社団法人 日本内分泌学会
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詳細情報 詳細情報について
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- CRID
- 1390282681270665728
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- NII論文ID
- 130000954198
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- NII書誌ID
- AA00635052
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- ISSN
- 21856370
- 00137219
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- NDL書誌ID
- 2475237
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- PubMed
- 6284496
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可